Micro: Dinucleotide

Dinucleotide AT-(Copy No.6)

Gene: Spacer between ML1749 and ML1750;IS1549 transposase and conserved hypothetical protein

AT AT AT AT AT AT

>Spacer
CGTTTGATTTTGTTGAGGGGGTGTCTGCGGTCTGGGTTCAGGTGAACGGTGTGGTGTGTTCGTTGTAGGT
GGGGATCGCGGTTTCGAGGTTGTTGATATCGGTGAACGTGTTGTGGCGGGTGATGGTGGTGAAGAAGATT
TTGGTTATGTGTCAGCTATGAGCAGCTTGTCGGTGTGAAGTGCAGTGTGGCTTGCTGGTTATCCTGTAGC
CAGGATCGGCTGCTGGGGGTTTGTGGGTTGGCGTAGTTGTCGCAGATTGGGGAATTGCACAAAACCGTGG
TCAAATACTGGCAGATCGACTAGATCCGTCCAGCGTTCATATATATATATGAAACTGCCACCAATTCTCA
ACCTCACAATCCCACCCTAATGCAATCGACGATAACGGTCAGGCAGAAACTACCAAAATTCTTGATCAGC
GCTTACAAACCCAAGCAAGTTTGGGGTCGGCGTTCGGGTTTTACGATCGGTGTATCTGGTGTTCGCTCGG
CTCCGGGCTTTGACATGTGCCAAGCGCCATGGGTATCACACCTTTTGCCAGCAGAGATTCCATCGCCGCA
CCGTCGACGGGACGTGAGAGCAGAAAGCCCTGCGCACGGTTGCACCCTAACTCCAGCAGCACCTGCGCTG





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Primers to amplify the repeat region

Product Size: 474bp

Forward:       GGTGAACGGTGTGGTGTGTT            Tm=61.03            GC%=55

Reverse:     CTTGGCACATGTCAAAGCCC            Tm=60.04            GC%=55