>ML1161 CCTCTTGCTTAGCAGCGTGATTCCTTATTCGTACTTTAGGAGTGGCATATTAATGATGTGAGCGACTGCT TGACTTGGAGATCGATAAGAATATAATTCATCTTCTTCCACTGGTATTGCTGGCCATTTATCTCATCGAG CACCCAAAACCTTTCGTAGGGATCCATACTCAACTTAGCCATATCTAACCTAGCCATGTCTAACCACAGC GTAGCTTACTCGGGACCAAGTGCTTTGAGTGTCACCACCCACCAACACGCAGTCTCCGGTAGCCGTATAG AATTTGCCGTATGCCAGCAATGCTTCGTTGTCCACATGATGGAAATGAATGGTGAGCAATTGCTGTAGTG TGGGATGGATTTTGCGAATTTTTTATCTACTTAATGAAATTCTCCAGCGATTTTCCTTCGACAAGCTCGC TAGCATAGTTGAGAGAGCGCAATTCTTATTTCTCTGAATCCAGCTATTCTTCGTTACCCTCCCGCACCGG GCGCTGCTCGAACAACTCGTAACCGGAGTACACACCTCAAGTCGGACTCATGGTCACCGCCAGCACTACG CGGATCGTGAACATGACTGGGCCATTATGGTGGAGCACTGCATGCAGGATGTCGCGGGTGTTGACGAACA GGTTCGGCCTGAGGTAGTCGGTGATTTCGTTGCCGAATTCGATGATTTCCCACTTGGCCGTACTCCAGGT GAAGTAGGTATAAGACTGCGTGAAGCCGAGCTTGGCCAGCCTGTATTGGCGTGCCGGAGACGTAAAAGCT TCGGATAAGAAGAGCACGTCGGGGTTGGTGATTGTGACCTTACAGATCAGCCAGGATCAGAAGTCGCGTG ACTTGGTATGCGGATTGTTGACTCGAAACAGCTTGACGCCGTGATTGATCCACTATAACACTACACGAAC CACTTCGTCATAGAGGCCGACAAGATCGTTCTCGAGGTTTAAGGGAGTAGATGTCCTGGTATTTCTTCGG ATGATTTTCCGCATAAGCTATTGTGCTATAAGGTAATTCGGTGAAACAATAACGATGTTGGCGTATCTAT GGATGATCTGACGAACATTGTAGCGCCAGATCCAGGGGCCATTTCCATTCCTAGTTTACTTGCCGCGGAA ACAAAGTCGTCGATAGTTTCCAGGCTTGGATGAATCACATCACATGGCCATCCTCATCGCTACCCATCGC CCATGGAGATCCGACATCTGTCGGTGCGGCGGTGGAGTAATTGTTGCGACCCTTGCGATGTATCTTGTCG AGCGGATGGATCGGCAGCAGGTACACCACGTTGAATCCCATGTCGGCTATACGCGAAGTACCGCGGCTGG GGTGGCGAAGGTGCCGTGTACCGGGTTACCGTCGGCGTCCCAGCAGCCGGTCGGGCGCGGAAACATTTCG TACCAGGAGCCAAAACGGGCCAACGGTCGATCCACCCAGACTCTGAACTGCTCGCCCCGGGTAACCAGAT CTTGCAGCGGATAATTCGTCAAAAGTTCATCGATTTCGGGCGCCAGTGCCAGCGTGGTGCGGGTCACCGG ATCTCTGGGGGCGTAACCTCGTCGAGGCCGTCAGCAGCAGGCCACGCAGCGCGCGATACACCGGTTGCGG CATGCTCGAACAGCGCTGCTCCGGCCAAAAGATCGTTGGACAATTCTGTCTTCACCCTGGTCGGCATGCA ACTTGACAATCAGCGCATGCCGCCAGGTGTGCATCGGATCGCCCCAGCTGCGTTGACTCGGAAGTTTCAC AATCCGACGCGGTCGGGGGGTGAACTGACCGTGAAAAACGTAGGGTTCTTGACACATCGTCATGTGAATC AGCAGCAACTCGACTCGTCGGACCTGGGTGTGGCTGCAGGACGTGCTTTGAGGTCCTGGACCACCTTGAT TCGGTGAGTTTTCGTCGCGAGCAGACAGCGAACCCGAGGTTGCGGACAACCAGTGTGGGGCGGGACGGCT TCGTGGCCTTCTCTCCATACCGTGGCGCTGACCGGGACCATTTCGCCGACTACGGCTTTAGCAGGGTACA TGCCGCAGGAGATGATCGGTGCGACGTTATGGATCTTGACCTGACCGGGCCACCACCACTCCTTCTG
The shaded nucleotide reflects the single nucleotide polymorphism in the repeat region
Primers to amplify the repeat region
Product Size: 250bp
Forward: TGTGAGCGACTGCTTGACTT Tm=59.90 GC%=50
Reverse: GAAGCATTGCTGGCATACGG Tm=59.97 GC%=55