Mini:15 bp repeats

15 bp Repeats-AAAGTTATAAATTAT-(Copy No:2.1)

Gene: Spacer between ML1730 and ML1731;alcohol dehydrogenase and ribonucleotide reductase small subunit

AAAGTTATAAATTAT

>Spacer GCGGGTAGATCTGTAGGCACAAGCCGTGACTCAACATAACACCCGGTTGGCCAACTGTCGGTAATGCAAG CGCTTTTCCTTGGAACCATCAACGGCTACAACAAAGTTATAAATTATAAAGTTATAAGTTATATTGTCCG
AGCTTTTCCAGCGGGAGCACTGGAACGTTTATTCCTAATGAATCTACAGTGTTTGGCTTGTCCCAACCAC


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The shaded nucleotide reflects the single nucleotide polymorphism in the repeat region

Primers to amplify the repeat region

Product Size: 208bp

Forward:      GCGGGTAGATCTGTAGGCAC            Tm=59.97            GC%=60

Reverse:     GGTTGGGACAAGCCAAACAC            Tm=59.90           GC%=55