Mini:15 bp repeats

15 bp Repeats-ACTACTCCTATTAAT-(Copy No.2)

Gene: Spacer between ML1829 and ML1830; hypothetical protein and putative aminotransferase(pseudogene)

ACTACTCCTATTAAT

>ML1944 ATGGTCCCTGAAGACGATACCGCCGCGATGCGCTCCACTACTCCTATTAATACTACACCTATTAATCAACTT
GGCTATGAAGCTTGCCGAGACGAGCTGATCGAAGTTGTGCGACTCTTGGAACAGGGTGGGCTAAATCTCGA
TATGTCGCTGAAGCTTTGGGAAAGAGGAGAACAGCTCGCTAAAAGATGTGAAGAGCACTTAGATGGCGCTC
GCCAGCGAGTCGCCGATGTGCTCTCCGCCAGGCAGGTCGAAGAAGCCTAA


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The shaded nucleotide reflects the single nucleotide polymorphism in the repeat region

Primers to amplify the repeat region

Product Size: 218bp

Forward:      GTCAGTCGATGATGCACCCA            Tm=60.11            GC%=55

Reverse:     CCCACCCTGTTCCAAGAGTC            Tm=59.96           GC%=60