Mini:19 bp repeats

19 bp Repeats-CAAGATTCAACGCTTGGAA-(Copy No:2.0)

Gene: Spacer between ML0404 and ML0405;cyclopropanemycolic acid synthase (pseudogene) and conserved hypothetical protein)

CAAGATTCAACGCTTGGAA

>Spacer ACTAGTTTGATAAATTTATTTTATGAGCCGGTAGCGAACGTCTATTGGACGACAGGCGTCACGTTCAGGC ATTGTTTCGCTGATGCGCAAGATTCAACGCTTGGAACAAGATTTAACGCTTGGAAGGGGAAAACT GTTTTAATTATGATCGAAGCAGATCTGCAGGCAGTATACGATTACGGTTATTCCATGTCGAGTCGATTTT GTACG


                                                      img

The shaded nucleotide reflects the single nucleotide polymorphism in the repeat region

Primers to amplify the repeat region

Product Size: 294bp

Forward:      CTTGGGTCTTGACTGGTGGT            Tm=59.53            GC%=55

Reverse:     ACTAGACAGCGCATCGTACA            Tm=58.91           GC%=50