>Spacer
ACCTCATCGCGGGAGATCGGGGACACGCGTGAAAGCCTACCTAGCCGCGAGCGTGAAGTTGGCCGCACAA
TAACTTTCTTTACCCAACAGAACTTTCTCTACCCAACAGAAAATCTAGATGCACGCTCGTCGGCA
GAGGAAGCCAGCCGGTCAGGCCTCTGGGTGGAAATGCCGCTTACCCAACGCTATGCAACAGTGTTGGCCGT
GCCT The shaded nucleotide reflects the single nucleotide polymorphism in the repeat region
                                                     
Primers to amplify the repeat region
Product Size: 233bp
Forward: TCAACACCAGTCGGGTTAGC Tm=59.97 GC%=55
Reverse: CACTGTTGCATAGCGTTGGG Tm=59.83 GC%=55