Mini:21 bp repeats

21 bp Repeats-TGTTGTCTTAGAGCATTGATT-(Copy No:2)

Gene: Spacer between ML0216 and ML0217;conserved hypothetical protein (pseudogene) and hypothetical protein

TGTTGTCTTAGAGCATTGATT

>Spacer CGATGATTCCAGGTGGCCTGGCGCAGCGCTGTCACAATAGGAGGTCTGGTAACGGGCAACTGCAGTGATC ACCATTTGTGCCCCACCTGTATCGTGCTCAGTATCACCATCGCTAGATTGAGGGGGTAAACAGCTACAAG CCCGGCTGCGGCCTTGGGTCAATGCGTCGGTGCTGTGCTGATCCGGTGTTCCCGGGTCGGCTCAGACACA CCTGTGTAGCTAGCTGCGAGAATCGGCGGTCAAAAGCCGTCGCGGCCGCCGCACCGTAAACCTCGGCCCT CCTGTTGTCTTAGAGCATTGATTTGTTGTCTTAGAGCATTGATTCCAATGAGACGGTAGTCAGTCC TTTGGACGAAAAAACGGCGTCGCCGGACGGTGTCCGAGTCTACCGCGCTTACTGCAGCGTCAACGGCGTTC GATGATGCGCTGTCGCAACCAGCCCATGCCGGTTGCCGATGCGCACAGCAGGCTACATGTCAACTTTAGTG ATCAACTCATTGTCAGTGAGCAAATCGATGCGGATAGTTATCAACTACATTGTTCCCCAATCGATTCTTTC TTCTGTCAGTTCATTAAGGTTCCAACCTGGGGAACTCTCTGGCCAAGTGCCTCAGAATCCGCTACCATTGT AGCTGGCCTCCACCTCCATGGGGATAGTTCTATTGCGGAGGCTGCATCGGCAGTAGTCTGCTTGAATCCG






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Primers to amplify the repeat region

Product Size: 613bp

Forward:      TGATCACCATTTGTGCCCCA            Tm=59.89            GC%=50

Reverse:     GATGCAGCCTCCGCAATAGA            Tm=60.25           GC%=55