Mini:21 bp repeats

21 bp Repeats-GTCGCCACTGACAACAGTAGT-(Copy No:2)

Gene: ML1002,possible conserved membrane protein

GTCGCCACTGACAACAGTAGT

>ML1002 GTGAGCAAGCGGGTAAGGAGGGCGCTGGCCAACACGGCGACCACAGCGGTGGTTACTGTGTTGTTGGCGC CTGTTGTGACTGCATCTCCCACCGGTGACGCCGACGATGCTATTACGGCCGCGTGGCACAAGGCCGGTGG TGACAATTCGGTGCTTGGCGCCAAGAAGGGCGAGGTATATCCTCTTGGTGACGGTTTCGCACAGAACTTT GCGTCCGGTAAACTATTCTTCACTACGGCTACCGGCGCCAAGTTGCTATATGGGCCAGTACTAGGTAAAT ACGAGTCACTCGGCGGTCCGGTCAACAGCGATCTGGGATTCCCAATCATCGACGAAGTTCCCGGTCTAGC TGGACCCGACAGCCGAGTTAGTATATTCGCTGCCAGCGACAACCCGGTGATTTTTTGGACGCCCGACCAC GGCGCGTTTGTTGTGCGTGGTGCGCTCAATGCGGCGTGGGACAAACTCGGCAGTTCAGCCGGCGTCCTCG GTGTCCCGGTCGCAGATGAAAGCTTCGACGGTGAGGTTATCTCCCAGAAGTTTAGTGGTGGCGCAGTCTT TTGGAACAAGAAAAGCAGCGAGTTCACCACCGAGCCAACGGCACTAGCCGAGCAGCTAACAGGATTGCTG GTAGCAACCGATCCCACCACGGCCATCAGCATGGCCTGGCGTGCGGCCGGCGGCGTTTCGGGTCCGCTAG GCCCCAAGCAGGGCGGGCAGCATCCCATCGGTGCTGACGGAATTATTCAGGACTTTGCTGGTGGCAAGGT ATTTTTCAGCAAAGCCACCGGTGCTAATGCGGTCGAGAATGACATTCTGGCTAAGTATGAGTCTCTAGGC GGTCCGGTCGGCAGCGACTTGGGTTTCCCCGCCGCCAACGAGGGAGACGGGGGCATCGTTACCTCCAGCC GGATCGCCATTTTCTCCGCAGCCGATAAGCCGGTGATTTTTTGGACACCCGACCACGGCGCGTTTGTCGT GCGCGGAGCGATGAAGGCGGCGTGGGACAAACTCAAAGGTCCCAACGGCAAACTCGGCGTGCCGGTAGCA GACCAATCGGTGGATGGTAATGTGATCTCGCAGAGATTTATCGGTGGCAAGATCTCATGGAACCGCGCTA ATAACACCTTCACTGCCGATCCGGCGAACTTAGCGTCGTTGTTGTCTGGTCTGCAGGTGTCCGAGCAGAG CCAACCGAGTGAGTTGGCGATGCTATTCCACGCCAAGAAATCCACCGGGCATTGGTGGTGGTTGGTGGCT GCGGTTGCTGTGCTGTTAGTGATCGTGATGTTCGTCTTGCTAGCACTCGGGTGGCGTCGGCGTCGCACCG CTCACGATATCGTTGCTGACTCCCATGACCACGATATGGACGTTGGCATGGACTTTGGCTACAAGGCCGC AGTTGATGGGCAGTGTACCTACGACGCCGAGTCTGGTACCGAGCATCCGGTGCGTGATGTCGAACGCGGA CCGACGGTCAGTTGGTTGCGTGGAGCACAGGACGCTACCGGCCTGGCTCAGGATGCTAGGCTTGGCCCGG CGTTCGGCAGCGAATTGCCGTCCGAGTCCGAGGAGCCTTTAGGCCTTGAAGAGGATGATCCCGACGCGGT GGATACGGATTCTATGCCGGCTCTGTCTGATGCTGACTTTCCATATGCTGCTTTGTCGGATGCAGCACAC TGGGATGCCGATGCTGCGGAGGTCGTCGCCACTGACAACAGTAGTGTCGCCACTGACAACAGTAG TTTGTTGGAAACTGCTGCTGACACTGAGGTAAGCACGGGTCGACACGCAGCCGTTGATATGGCAGATGCG ATGGATGCGTCCTTCCCGGGCGTCACTGTGCCGAGGATGCTGTTCCGGCCAAGCCCGACTCTCCGGCCGA CGATCCACCTGCCGTTGGATGACCCTTATCAGGTACCCGACGGTTATCCTATTAAAGCTAGTCCGCGCTT CGGTCTGTACTATACCCCGGCGAGCGAATTATACCATGACACGCTCGCTGAAGTCTGGCTAGCCAATGAA GAAATCGCCCAGGTCAACGGCTTTATTAAGGCCGACTGA


















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Primers to amplify the repeat region

Product Size: 416bp

Forward:      CGAGTCCGAGGAGCCTTTAG            Tm=59.62            GC%=60

Reverse:     TCGCCGGGGTATAGTACAGA            Tm=59.53           GC%=55