Mini:21 bp repeats

21 bp Repeats-CTAGAGTTGTCAGTCACAGAA-(Copy No:2)

Gene: Spacer between ML1189 and ML1190;hypothetical protein and conserved hypothetical protein

CTAGAGTTGTCAGTCACAGAA

>Spacer CTCGCCACCTTGCCGCCATTCCGGTGCTAGCTGCATCCGGAGATGGCGAGTTGTTTCGCGATCTGCGCGC TTCTTTCAACGGCGACGATGGATAGCGATTGGTGACTGTCGATCACGAAATCACTGTCGAAGGAGTCCAC GCGGTACGATGTTGTGTCGCAGCGCATACGGCGATACGACTCTGCAGGCCACAGGGGTATTTTGGGATTA ACTGAATTTACGAGCGCGGTGGCTGGTATTTCTCTTGCCGCAATTTTGCCTGGGATGCGGTACCAAGCCG TCATCCGCTCGGTCATGTGCGCAGTACCGCTACGTTGCTTGACATGAAAGGGATTTAGCGCTGCTATCCA TTTCAGTTAGTACCCAAGTATTGGAAGCTAGAGTTGTCAGTCACAGAACTAGAGTTGTCTGTCAC AGAAGGTGTCCACCCCGACCAGGAGTACGGTACTTGGCTCATGGCTGGCCACCTCGACGCTGCCGAGATC GTTCAGTCATTTGCCTTTCTGAGGATATTCAATTAAGAATCGGCCCGGTGGTACCGATAGTCGCCGGTTT CTATACGATGGTGCTATTTTATCTACAGACCGGTGATTACGGCACCTATAAAGGATTATGGCATACCGCA GGCCACCCTGAATGCGGACATACAGCGCAATTTGGCTACCATCGTATGGCTGCCTAGGTCTTGAATATCA AAGCGGAGCCAGAGCTGCGTCGTGGGCTGTCCACTGCGACGGTGACGAACATTACTTCCTTGTTCCTGCC AAGTT







                                                      img

The shaded nucleotide reflects the single nucleotide polymorphism in the repeat region.

Primers to amplify the repeat region

Product Size: 497bp

Forward:      ATTTTGCCTGGGATGCGGTA            Tm=60.03            GC%=50

Reverse:     TAATGTTCGTCACCGTCGCA            Tm=60.04           GC%=50