Mini:21 bp repeats

21 bp Repeats

Gene: Spacer between ML2307 and ML2308;hypothetical protein and penicillin binding protein (class A)

TAGGAGCCAACCAGCGACGCG

>Spacer CACTCCTGCATTACCGGGCAGTGACGACAGATCACCGCGGCTTTACGTTGAGCGGCACCCCGGACAAATA ACTCATCCGGATCCGTCGCCCGACACAACGCTTTGGAAACCCAGGCAATGCGCTCCTCGGCGTCGACACT ATGGAGAGGACTCTTGGCGGCGGACAGGTTTGTTCTACGGGCGGCCGGTCGTGTTTTTGCCACGAGCTGT TACCTTCCTCCCGGCCGCAATTCACGGCCGCCTTCCTCTGGTGAAGACCGTTATGCGATCTACGCCACAC CATCTTCATAGTTGTTATATATCTCACTGTGAGTCTACAGTTAAGTGTGACCAGAGGCCAATTGCACAAC AATCTGATAACGCTTTTTTAAGGACAAGCGTTCCTTCCCGACGTGATCATGGTAAGGAGCACTGCGCACC ACTTTTAGGAGCCAACCAGTGACGCGTAGGAGCCAACCAGCGACGCGCACGAAAACTTCGGTGCT GATCATCGACAAAGTTGCTGATAACCAGTCGCTCAAGATTTCTATGCGACTTCACTGCTAGTCGCCACAA CTGGGGGCTACCGTAGTACACATGTCAGAGCGCCTCCCAGCCGTCCTCGCAGTTCTCAAGCTTGCGGGGT ATTGCCTATTGGCCGGCGTTGTCACCACCGCAATGATGTTCCCGTTGGCCGGCGGACTGGGGGTGATATC CAACCGTGCTTCGGAAGTGGTAGCCAACGGATCGGCCCAACTTCTCGAGGGCGAGGTGCCCGCGGTATCG ACAATGGTCGACGCGAAGGGAAACACGATCGCGTGGCTGTACTCGCAACGCCGATTCGAGGTACCCACCG ACAAG







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The shaded nucleotide reflects the single nucleotide polymorphism in the repeat region.

Primers to amplify the repeat region

Product Size: 356bp

Forward:      TATGCGATCTACGCCACACC            Tm=59.97            GC%=55

Reverse:     AGCTTGAGAACTGCGAGGAC            Tm=60.04           GC%=55