Mini:22 bp repeats

22 bp Repeats

Gene: Spacer between ML1581 and ML1582;conserved hypothetical protein and probable integral membrane protein

TTTCTCGGTTTTCACTATTGGC

>Spacer TCACCGACCCGGCCGTCGAATTCCTTGATGTTGCCGGGGTTGACCCGCACCGCGGCACACCCGGCGTCAA TGGCGGCAAAGATGTATTTCGGCTGGAAATGTATGTCGACAATCACCGGAATCTTGCTGTGCCGGGCGAT TTCGGCAAGTGCGTCAGCATCCTCTTGACGCGGGCAGGCCACCCGAACGATGTCACAGCCGGCAGTGGTC AACTCCGCAATCTGCTGCAACGTCGCATTGACGTCATGAGTCTTGGTGATGCACATCGACTGCACGGAGA TCTGGTGATCACTACCGACCCCAACGTTGCCGACCATCAATTGACGCGTGATACGCCGGGGGGCAAGCCC GGGAGCCGGAGCCTCGGGCATGCCCAGACGCATGTTCACTGTTCCCCATTTCTCGGTTTTCACTATTG GCTTTCTCGTTTTTCACTATTGGCTATTGAAAAAGCCTGATTGGGTTTACCAGATCGGCGGTCACGGT CAACAGCACATACCCGACTACAAAGACCAAGACCACATAGGTCGCGGGCATGAGCTTGAGATAGTTCACCG GCGCGGCCACGACCACGCCACGTGCGGACCGGATGAGGTTACGGATCCTCTCGAATACGGCAATGGCGATG TGGCCGCCATCGAAAGGCAGCAACGGCACCAGGTTGATGGCACCCAGAATAAGGTTCAGCTGCGCCAAGAA AAACCAGAACGCCACCCACAGCCCATGATCGACGGTGTCGCCGCCGATGATGCTGGCGCCGACCACACTCA







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The shaded nucleotide reflects the single nucleotide polymorphism in the repeat region.

Primers to amplify the repeat region

Product Size: 217bp

Forward:      GATCACTACCGACCCCAACG            Tm=60.18            GC%=60

Reverse:     TCGGGTATGTGCTGTTGACC            Tm=60.04           GC%=55