Mini:52 bp repeats

52 bp Repeats

Gene: ML0057,conserved hypothetical protein(pseudogene)

TTGTTGTTGCCAACATTGCGACGCCACTACCGAGTCTCCACTCCCGGTCCGG

>ML0057 ACCTCTGGAAGGAGTGTCGTAAACGTGATCTAACCAGGAGCCCGCTGGTAGCAGCTCCATCAGGTTGTTG
AAGGCGACCTGTAGGTTTTGCTGAATGCCCGACAGGAAGCTCCCGTACAGTTCGTTACCAACCCGACGGG
GCAGCGCGACACCAACCTGCCCTGCGCGGAGTCGAGAACCCCTTACAGCTGTGTCCGTGTATGTTGTCGT
GCCTCCCGGCGGGCGTTGAGTAACGATGATCGCCAACCAGTTGGACGGGTTCCTGAGGAACTTGCCATAG
ATGCAGGCTGAGCTCGGTTGGACACCTTGTCCGACCAACTGGGCTGCGGTTCTGTACTCGGCTAGTTTTT
TGGCTATGCCGGTCAGCGGTTGGACATTTGCAGACTCTGCAGGGTCCAGCATGGTGGTCACCATGCTGGC
CTGGCTGATCTGTAGAGCCATTAACTGTAGTATCAGCATGTCGTCCTATCGCGCGCCGCGATGACGACAT
GGCGGTCGCCCCTGCGGCACACAACGAAGGTGCACCATTTGTTGTTGCCAACATTGCGACGCCACTACCG
AGTCTCCACTCCCGGTCCGGTTGTTGTTGCCAACATTGCGACGCCACTACCGAGTCTCCACTCCCGGTCCGG
CCGGTTAGACGTATCTGCCATCGCTGCCAACTTCGGGTAAGGTTGGCCGTTAGCGTCCCTGAATTCCCTG
GGAGGCAAGGTCACATTGCACCCGTTTCCAGACCACGGCTGGTAGTTCCTGTCTGAGGAATCATTGATTC
GTATCCCGAGTGCCGTAGGGAAAGCTACAAGGTGCAATCGGTCCGTGACTACCAACATATAACATACCGT
CAACATACCGTCGGTAGTCACTTCGACGCCGACCACATCGTTGACGAGGCCAGCGCGACCGGAAGCCAGC
GGACCGGACAT








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Primers to amplify the repeat region

Product Size: 304bp

Forward:      CTGGCCTGGCTGATCTGTAG            Tm=59.89            GC%=60

Reverse:     AATGTGACCTTGCCTCCCAG            Tm=59.96           GC%=55